rabbit anti rat pdgfrbeta  (Bioss)

Journal: Kidney360

Article Title: Natural Killer Lymphocytes Mediate Renal Fibrosis Due to Acute Cardiorenal Syndrome

doi: 10.34067/KID.0000000000000305

Figure Lengend Snippet: Kidney mesenchymal cells expand after CA/CPR. (A) Representative flow cytometry plots of PDGFRβ+ mesenchymal cells in the kidney after CA/CPR. Plots shown are gated on live, single cells. (B) Quantification of (A). (C) Representative flow cytometry plots of CD45+PDGFRβ+ mesenchymal cells in the kidney at different time points after CA/CPR. (D) Quantification of (C). (E) Representative 20× immunofluorescence images of PDGFRα/β staining at different time points after CA/CPR. (F) Representative 20× immunofluorescence images of αSMA staining at different time points after CA/CPR. (G) Quantification of (F). (H) qRT-PCR for Acta2 (codes for αSMA) and Col1a1 (codes for type 1 collage) in the kidney at 49 days after CA/CPR compared with sham. *P < 0.05, ***P < 0.001 compared with sham in one-way ANOVA. n=4–8/group. Scale bars=100 μm. CA/CPR, cardiac arrest/cardiopulmonary resuscitation; #αSMA+, number of αSMA positive cells per high-powered field; αSMA, α smooth muscle actin; PDGFRα/β, platelet-derived growth factor α and β.

Article Snippet: Sections were then incubated with primary antibody (rabbit anti-PDGFR α / β , Abcam ab32570, 1:1000, and rat anti-CD45, BD Biosciences 550539, 1:1000) in 1% bovine serum albumin in 0.01 M PBS and 0.1% Triton X-100 at 4°C overnight.

Techniques: Flow Cytometry, Immunofluorescence, Staining, Quantitative RT-PCR, Derivative Assay

Journal: Kidney360

Article Title: Natural Killer Lymphocytes Mediate Renal Fibrosis Due to Acute Cardiorenal Syndrome

doi: 10.34067/KID.0000000000000305

Figure Lengend Snippet: Immune cells colocalize with mesenchymal cells during AKI-CKD transition. Representative 40× immunofluorescence images of different time points after CA/CPR, including 3 days (A), 7 days (B), and 49 days (C). CD45 used to label immune cells, and PDGFRα/β used to label mesenchymal cells. Scale bar=100 μm.

Article Snippet: Sections were then incubated with primary antibody (rabbit anti-PDGFR α / β , Abcam ab32570, 1:1000, and rat anti-CD45, BD Biosciences 550539, 1:1000) in 1% bovine serum albumin in 0.01 M PBS and 0.1% Triton X-100 at 4°C overnight.

Techniques: Immunofluorescence

Journal: Journal of Cellular and Molecular Medicine

Article Title: VEGFR2 + PDGFRβ + circulating precursor cells participate in capillary restoration after hyperoxia acute lung injury (HALI)

doi: 10.1111/j.1582-4934.2009.00785.x

Figure Lengend Snippet: Phenotypic characterization of CPCs. LSR-II flow cytometric analyses of (gated, a) mononuclear cells in peripheral blood, identified a distinct population of CPCs that co-express VEGFR2 and PDGFRβ (c); single-colour controls were used for compensation ( e.g. see staining for PDGFRβ-Alexa Fluor 647 in b). These CPCs (in blue) are positive for CD45 (d) and show scattering properties typical of small cells with a high nucleus to cytoplasm ratio (a, d). A subset of CPCs is positive for CD11b (e), whereas all VEGFR2 + PDGFRβ + CPCs are positive for CD31 (f); CPCs are distinct from cells with a phenotype consistent with ‘circulating ECs’, i.e. CD31 + CD45 − (see gated population in f).

Article Snippet: All Unicryl sections were pre-treated with 1% bovine serum albumin (BSA) in PBS (5 min at RT), incubated (overnight at 4°C) with ( i ) mouse anti-rat monoclonal antibody to CD11b (Calbiochem, La Jolla, CA, USA); ( ii ) rabbit anti-rat polyclonal antibodies to VEGFR2 or PDGFRβ (Calbiochem); or ( iii ) a rabbit polyclonal antibody to von Willebrand factor (vWF, DAKO, Carpinteria, CA, USA), rinsed in PBS, and incubated (60 min at RT) in protein A-gold (pA-AU) (10-nm diameter, Auroprobe AG10, Amersham, UK) diluted 1:50 in PBS prior to double-staining.

Techniques: Staining

Journal: Journal of Cellular and Molecular Medicine

Article Title: VEGFR2 + PDGFRβ + circulating precursor cells participate in capillary restoration after hyperoxia acute lung injury (HALI)

doi: 10.1111/j.1582-4934.2009.00785.x

Figure Lengend Snippet: Phenotypic characterization of CPCs in lungs post-HALI. Antigenic sites, visualized with 10-nm protein A-gold by high-resolution microscopy, demonstrated that CPCs were VEGFR2 + (a) and PDGFRβ + (b) post-HALI (weeks 6 and 7). Representative images of the same CPC profile in adjacent sections of lung tissue (c and d, and insets) demonstrated co-expression of VEGFR2 and PDGFRβ antigenic sites post-HALI (week 7). Other representative images of additional antigenic sites expressed by CPCs post-HALI (week 7): CD11b (e and inset) and for vWF (f). Typically, the sites for CD11b were uniformly distributed over CPCs but also appeared as clusters (e, see bottom inset, illustrating an example of a cluster of ∼40 CD11b + labelled sites). Ten-nm gold-labelled antigenic sites (a, c–f) are circled for clarity to distinguish these from 7 to 8 nm ribosomes on cell profiles. Ninety-nm-thick Unicryl resin sections stained with uranyl acetate and lead citrate. Bars = 0.5 μm (a–f).

Article Snippet: All Unicryl sections were pre-treated with 1% bovine serum albumin (BSA) in PBS (5 min at RT), incubated (overnight at 4°C) with ( i ) mouse anti-rat monoclonal antibody to CD11b (Calbiochem, La Jolla, CA, USA); ( ii ) rabbit anti-rat polyclonal antibodies to VEGFR2 or PDGFRβ (Calbiochem); or ( iii ) a rabbit polyclonal antibody to von Willebrand factor (vWF, DAKO, Carpinteria, CA, USA), rinsed in PBS, and incubated (60 min at RT) in protein A-gold (pA-AU) (10-nm diameter, Auroprobe AG10, Amersham, UK) diluted 1:50 in PBS prior to double-staining.

Techniques: Microscopy, Expressing, Staining